Diagnostic description

According to university of Adelaide and Iranian Association of Clinical Laboratory Doctors, there are different ways  to diagnose this infection:

1-Direct Microscopy: using 10% KOH and parker ink or calcofluor white. Packed mass of brown pigmented branching hyphae can be observed surrounding the asci, each of fungus holds eight ascospores.

2- Culture: using primary isolation media such as Sabouraud's dextrose agar. The fungus will produce after 2-3 weeks a dark brown to black colonies with folded surface and a flat margin (Figure 2) (4-7)

Figure 2: black colonies are white folded surface and a flat margin